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Tetraalkylammoniums (YK-421)

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Trialkylammonium were separated using cation chromatography column,
IC YK-421. By using the mobile phase used below (high ACN concentration), hydrophobic interaction is suppressed. Thus ioninc-interaction dominates, this makes shorter chain alkylammonium to be retained longer than longer ones.

Sample : 100mg/L each, 20?L

1. Tetrahexylammonium

2. Tetrapentylammonium

3. Tetrabutylammonium

4. Tetrapropylammonium

5. Tetraethylammonium

6. Tetramethylammonium

 Column : Shodex IC YK-421 (4.6mmID*125mm)
 Eluent : 2.5mM HNO3 aq./CH3CN=30/70
 Flow rate : 1.0mL/min
 Detector : Shodex CD
 Column temp. : 40°C