Tetracaine was analyzed using Asahipak ODP-50 4D (a column for reversed phase chromatography) with eluents of different pH values.

Sample : Tetracaine hydrochloride

 Column : Shodex Asahipak ODP-50 4D (4.6mmID*150mm)
 Eluent : 25mM Phosphate buffer/CH3CN
 Flow rate : 0.6mL/min
 Detector : UV(254nm)
 Column temp. : 30°C