IEC DEAE-825 packed with porous gel can allow larger sample loads than IEC DEAE3N-4T packed with non-porous gel.
Transfer ribonucleic acid, tRNA (from E.Coli)
Column : Shodex IEC DEAE-825 (8.0mmI.D. x 75mm) Eluent : (A); 20mM Sodium phosphate buffer(pH6.6) + 250mM KCl + 5M Urea + 0.5mM EDTA (B); (A) + 250mM KCl Linear gradient: 0min to 150min, (A) to (B) Flow rate : 1.0mL/min Detector : UV(260nm) Column temp. : Room temp.