The ligand of AFpak AAB-894 (an affinity column) is aminobenzamidine (serine protease inhitor). The column was used for the analysis of trypsin. Trypsin was adsorbed by the ligand when the eluent of pH 7.0 was flowed and it was released from the ligand when the eluent of pH 2.8 was flowed.
Sample : Trypsin
Column : Shodex AFpak AAB-894 (8.0mmID*50mm)
Eluent : (A); 0.1M Sodium acetate buffer(pH 7.0) + 0.5M NaCl
(B); 0.1M Sodium acetate buffer(pH 2.8) + 0.5M NaCl
Step gradient: (A) to (B)
Flow rate : 1.0mL/min
Detector : UV(280nm)
Column temp. : Room temp.