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Ultra-rapid Analysis of Hemoglobin A, F, S and C (SP-FT 4A)

Hemoglobin A, F, S and C were analyzed using IEC SP-FT 4A, a non-porous type column for cation exchange chromatography. SP-FT 4A can provide ultra-rapid and high-resolution analysis using conventional devices as same as when using UHPLC.
(Note) Total system pressure (system pressure and column pressure) varies depending on the system, solvent composition, temperature, and gradient conditions used. Use the column within the stated total system max pressure.


Sample : 5μL
AFSC Hemoglobin Control
(51-fold diluted)

 

Column       : Shodex IEC SP-FT 4A (4.6mmI.D. x 10mm)
Eluent       : (A); 20mM *MES buffer(pH5.6)
               (B); (A) + 0.5M Na2SO4
               Linear gradient: 5% (A) to 100% (B) (2min)
Flow rate    : 1.7mL/min
Detector     : VIS(415nm)
Column temp. : 30°C
*MES : 2-(N-Morpholino) ethanesulfonic acid