The 2020 Events List. Details available throughout the year.
Pittcon 2020: Complete. Thanks for visiting our booth this year. Please feel free to contact us if you need poster reprints or other technical assistance.
The 68th ASMS Conference: The 2020 Reboot. Look for our poster in the event planner. See the title below.
MP 322 LC/MS analysis of phosphorothioate oligonucleotides using a polymer-based HILIC column having diol
group; Leah Sullivan; Shodex, Showa Denko America, Inc., New York, NY
We will only attend these two shows this year due to COVID-19 health issues.
Event Venue:George R. Brown Convention Center | Houston, Texas
Please visit our on demand poster via the online planner, Monday, June 1
MP 322 LC/MS analysis of phosphorothioate oligonucleotides using a polymer-based HILIC column having diol group; Leah Sullivan; Shodex, Showa Denko America, Inc., New York, NY
Event Venue:McCormick Place Chicago, Illinois, USA
Visit us at Booth 1833 and discover the newest technologies offered by Showa Denko, manufacturer of Shodex LC columns. Learn more at http://www.pittcon.org.
(790-2 P) Development of GFC Column for Antibody Drug Analysis by LEAH BLOCK, Shodex, Showa
Denko America, Junya Kato, Hirotsugu Nakanishi, Asuka Toda, Ron Benson
Poster 3. Characterization of Polymers and Plastics
(2030-2 P) Rapid Measurement of Molecular Weight by a Novel GPC Column by LEAH BLOCK, Shodex,
Showa Denko America, Junya Kato, Hideyuki Kondo, Naoko Maruoka, Ritsuko Wakayama,
Ron Benson
Event Venue:Georgia World Congress Center in Atlanta
Visit us at Booth 3428!
Come see our Posters
Abstract Number:
750 – 9
Author Name:
Ronald Benson – Shodex/Showa Denko America, Inc.
Session Title:
Commercial Products Characterization
Event Type:
Poster Sessions
Event Title:
High Sensitive LC/MS Analysis of Stevia Sweeteners Using Polymer-Based Amino Column
Co-Author:
Junji Sasuga, Satoko Sakai, Tomokazu Umezawa
Affiliation:
Shodex/Showa Denko America, Inc.
Date:
Monday, March 7, 2016, presenters present 1 pm to 3 pm
Start Time:
(Slot #9)
Location:
Exposition Floor, 400 Aisle
Abstract
In recent years, such as Rebaudioside A, a glycoside derived from stevia, is added to a variety of processed foods as a sweetener for reduced calories. It is important to quantify the stevia sweeteners in processed food for quality control purposes. Herein we describe the highly sensitive and highly selective analytical separation by using polymer-based amino column with detection by LC/MS. Chromatographic separation was conducted using polymer-based amino column with isocratic mixture of (A) 0.1% NH3 aq. / (B) CH3CN as the eluent. The flow rate was 0.2mL/min and the temperature was 30 °C. ESI was selected for ionization method and SIM (-) for detection mode. Eight components, Rebaudioside A/B/C/D/F, Isosteviol, Stevioside, Steviolbioside, were mixed in each 50ng/mL as a standard sample. Injection volume was 5μL. Eight components were almost separated within 20 minutes and were detected with sufficient S/N in the condition of 78% CH3CN by HILIC mode. Rebaudioside B and Steviolbioside were strongly adsorbed to the column when the eluent (A) was only water since they have a carboxyl group. It became possible to adjust the appropriate retention time by alkaline with NH3. Result of measuring commercially available energy drink containing stevia sweeteners as a real sample, Rebaudioside A was detected, and additionally the other additives such as Sucralose, Acesulfame, Erythritol, Carnitine, Leucine, Isoleucine, Varine and Pyridoxine were also detected at the same time with good peak shape. The analysis condition show that the stevia sweetener in processed food and other high polarity food additives can be separated by HILIC mode and quantify with high sensitive.
Abstract Number:
1750 – 11
Author Name:
Ronald Benson – Shodex/Showa Denko America, Inc.
Session Title:
Liquid Chromatography
Event Type:
Poster Sessions
Event Title:
Investigation of Reverse-Phase – HILIC Continuous Analysis Using a One Column
Co-Author:
Junji Sasuga, Tomokazu Umezawa, Hideyuki Kondo
Affiliation:
Shodex/Showa Denko America, Inc.
Date:
Wednesday, March 9, 2016, presenters present 10 am to noon
Start Time:
(Slot #11)
Location:
Exposition Floor, 400 Aisle
Abstract
A sample matrix that includes both hydrophobic and hydrophilic substances are difficult to analyze by reversed phase chromatography (RPC) or hydrophilic interaction chromatography (HILIC) alone. The analysis of such matrices requires the separation using both an RPC column and a HILIC column. Herein, we describe the method for analyzing both hydrophobic and hydrophilic compounds simultaneously with one column by applying RPC/HILIC continuous analysis method.
First, the transition in the retention behavior of hydrophilic compounds (Cytosine, Uridine and Uracil) and hydrophobic compounds (Toluene and Naphthalene) corresponding to changing CH3CN ratio was examined by using CH3CN / H2O as eluent. Chromatographic separation was conducted using RPC column (4.6mmI.D. ×150mmL) packed with polyhydroxymethacrylate. As a result, the retention order was reversed in two conditions, 30% CH3CN and 90% CH3CN. It is suggested that the RPC mode acts in low CH3CN concentration and the HILIC mode act in high CH3CN concentration. By taking account of other conditions, it is confirmed that the RCP mode appears in under 50% CH3CN condition and HILIC mode appears in above 70% CH3CN condition.
Second, the analysis for a commercially available energy drink by LC/MS with gradient condition from RPC mode to HILIC mode continuously was attempted. As a result, the method that both hydrophobic and hydrophilic compound can be separated in a single measurement was established with twice sample injection. This method realizes quick equilibration as switching the mode and rapid analysis.
The polymer-based RPC column containing hydroxyl group which was considered this time has a unique performance that can be applied to HILIC mode as well. This column realizes efficient analysis for hydrophobic compound and hydrophilic compound in a single measurement.
La Asociación Farmacéutica Mexicana, A.C. Celebra una vez más junto con el apoyo de todos ustedes la mejor e importante exposición de México y Latino América. En este 2015 nos complace celebrar el 42 aniversario de este tan importante evento, por lo cual es para nosotros un honor invitarlo a participar nuevamente en la cuadragésima segunda edición. de EXPOFARMA 2015
Event Venue:Ernest N. Morial Convention Center, New Orleans, LA
March 8 – March 12
“Pittcon is the world’s largest annual premier conference and exposition on laboratory science. Pittcon attracts more than 16,000 attendees from industry, academia and government from over 90 countries worldwide.” Read and find out more at Pittcon.org.
Please visit Shodex Booth 3146 at Pittcon 2015 in New Orleans! Find out the latest news and about our new product offers.
